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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

This restoration of cortical glutathione is crucial for neutralizing the neurotoxic reactive oxygen species that damage delicate neural pathways and impair cognitive function

For a 5 mg vial, add 2.5 mL bacteriostatic water to create a 2 mg per mL concentration

The biology after 50 is not the biology at 40, and a protocol designed for a perimenopausal patient is not automatically right for a post-menopausal patient fifteen years past her last cycle